Coding

Part:BBa_K1819001:Design

Designed by: Lais Ribovski   Group: iGEM15_Brasil-USP   (2015-09-13)

Latex Clearing Protein (Lcp) - rubber degradation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 889
    Illegal XhoI site found at 1043
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 535
    Illegal NgoMIV site found at 857
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 438


Design Notes

Because Lcp is secreted by Streptomyces sp, it contains a 5’-signal peptide that was identified using signal peptide predictor server.[1] We removed this region from Lcp to generate this part flanked by SacI and NdeI restrictions sites

Source

Lcp from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1).

References

1. Bagos PG, Nikolaou EP, Liakopoulos TD, Tsirigos KD. Combined prediction of Tat and Sec signal peptides with Hidden Markov Models. Bioinformatics. 2010; 6(22):2811-2817.